Altered plastid levels and potential for improved fruit nutrient content by downregulation of the tomato DDB1-interacting protein CUL4

Fruits are a major source of nutrition in human diets, providing carbohydrates, fiber, vitamins and phytonutrients. Carotenoids are a principal class of compounds found in many fruits, providing nutritional benefits both as precursors to essential vitamins and as antioxidants.

Molecular characterization revealed that the tomato high pigment mutant genes (hp1 and hp2) encode UV-DAMAGED DNA BINDING PROTEIN1 (DDB1) and DE-ETIOLATED-1 (DET1) homologs, respectively, and both are essential components of the recently identified CUL4-based E3 ligase complex. Here we have isolated a tomato CUL4 homolog and performed yeast two-hybrid assays to suggest possible association of tomato DDB1 with CUL4 and DET1.

Real-time RT-PCR analysis indicated that both HP1 and CUL4 are expressed constitutively. Abscisic acid is implicated in plastid division control and its application substantially enhances HP1/DDB1 mRNA accumulation.

Transformation of constructs expressing CUL4-YFP and DDB1-YFP fusion proteins driven by the CaMV 35S promoter reveals that both CUL4 and DDB1 are targeted to tomato plastids and nuclei simultaneously. Using fruit-specific promoters combined with RNAi technology, we show that downregulated DDB1 expression in transgenic fruits results in a significant increase in the number of plastids and corresponding enhanced pigment accumulation.

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EUR 165
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EUR 422
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G-749

HY-12333 25mg
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HY-12588 50mg
EUR 601

Neuropeptide g

H-7455.0500 0.5mg
EUR 181
Description: Sum Formula: C99H158N34O29S; CAS# [114882-65-4] net

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G-5555

HY-19635 50mg
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HY-N0855 10mg
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Gomisin G

HY-N0858 10mg
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Tenacissoside G

HY-N2103 1mg
EUR 179

Hosenkoside G

HY-N2242 1mg
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Rebaudioside G

HY-N2291 1mg
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Saikosaponin G

HY-N4216 1mg
EUR 443

Kuwanon G

HY-N4247 10mg
EUR 436

G-1

HY-107216 10mg
EUR 360

g-Endorphin

H-2725.0001 1.0mg
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g-Endorphin

H-2725.0005 5.0mg
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Tenacissoside G

N2128-20 20 mg
EUR 282
Description: Extracted from Marsdenia tenacissima (Roxb.) Wight et Arn.;Store the product in sealed, cool and dry condition

Cathepsin G

MO20021 100 ul
EUR 213

CUL4-RNAi repression lines provide insight regarding CUL4 function during tomato development, and reveal that this tomato cullin is important in the regulation of plastid number and pigmentation, which in turn have a direct impact on fruit nutrient quality.

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